FIGURE 5.

Computational modeling and effect of mutagenesis on Cdc37 residues. A, interface residues of Hsp90-Cdc37 complex. Cdc37 is shown as schematic in yellow with Hsp90 in surface representation. Cdc37 residues selected for mutation and polar Hsp90 residues that interact directly are shown in stick representation. B, luciferase assay for Cdc37-CRL mutations. FL was used to normalize the transfection efficiency. Luciferase assay was performed as described under “Experimental Procedures.” Data are presented as means ± S.D. (n = 3).