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. 2010 Apr 28;285(27):20975–20981. doi: 10.1074/jbc.M110.102210

FIGURE 3.

FIGURE 3.

Heterodimers of αNR2A-βNR2B preferentially traffic through recycling endosomes. A, heteromeric αNR2A-βNR2B dimers preferentially sort to recycling endosomes. Cells expressing αNR2A-βNR2B and GFP-Rab9 or GFP-Rab11 were labeled with MHC II αβ-dimer (L243) antibody on ice for 45 min and returned to conditioned media for 30 min at 37 °C. The cells were fixed and permeabilized, and the immunoreactivity was visualized with Alexa Fluor 568-conjugated (red) secondary antibody. Images were collected using a 63 × objective at intervals of 0.34 μm. Projection images are shown. Arrows indicate colocalization. B, statistical analysis of the amount of colocalization between αNR2A-βNR2B dimers with GFP-Rab9 or GFP-Rab11. Colocalization analysis was measured with the software NIH ImageJ Overlap coefficient plug-in. Values represent the mean ± S.E. (error bars) of overlap coefficient (n = 17–25, n = 4). Student's t test was performed; *, p < 0.01.