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. 2010 May 3;285(27):20904–20914. doi: 10.1074/jbc.M109.098558

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — MLL5 displays slower gel mobility at G₂/M transition. A, expression of MLL5 in HeLa cells released from the G₂/M boundary. B, expression of MLL5 in asynchronous (no treatment (NT)) and G₂/M-arrested HeLa, U2OS, and HCT116 cells. G₂/M synchronization was achieved by treatment with nocodazole (Noc), vinblastine (Vin), or taxol (Tax). C, expression of MLL5 in HeLa cells that were released from G₁/S synchronization. Cyclin A, cyclin B1, and phosphohistone H3^Ser10 serve as cell cycle progression markers. Actin serves as a protein loading control. Arrows indicate MLL5, and arrowheads denote the slower migrating form of MLL5 at G₂/M phase.