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. 2010 May 3;285(27):20904–20914. doi: 10.1074/jbc.M109.098558

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FIGURE 2. — MLL5 is phosphorylated at Ser/Thr residues at G₂/M phase. A, alkaline phosphatase treatment of G₂/M-arrested HeLa cell extract converted MLL5 to a faster migrating form that ran to the same migrating position as in the asynchronous cell extract. CIP, calf intestinal alkaline phosphatase. B, endogenous MLL5 was immunoprecipitated (IP) from asynchronous or G₂/M-arrested HeLa cell extracts. Phosphorylated MLL5 (arrowheads) is present at G₂/M phase and can be detected by anti-phospho-Ser/Thr but not anti-phospho-Tyr antibodies.