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pH dependence of kinetic parameters for the SDH E122A mutant enzyme in the direction of saccharopine formation. Data were obtained at 25 °C for V2/Et (A), V2/KLysEt (B), and V2/Kα-KgEt (C). The points are the experimentally determined values, whereas the curves are theoretical based on fits of the data using Equation 8 for C. The curve for B was drawn by eye, and an average value is given for A.
