. 2010 Apr 28;285(27):20756–20768. doi: 10.1074/jbc.M110.119826

TABLE 1.

DNA sequences of the forward and reverse PCR primers

Primers^a	DNA sequence from 5′ to 3′
E78Q Forward	`AGAATCATTATAGGTTTGAAGCAATGCCTGAAACCGATACTTTC`
E78Q Reverse	`GAAAGTATCGGTTTCAGGCATTGCTTCAAACCTATAATGATTCT`
E122Q Forward	`CGGTACTCTATATGATTTGCAATTTTTGGAAAATGACC`
E122Q Reverse	`GGTCATTTTCCAAAAATTGCAAATCATATAGAGTACCG`
E78A Forward	`CATTATAGGTTTGAAGCAAATGCCTGAAACCG`
E78A Reverse	`CGGTTTCAGGCATTTGCTTCAAACCTATAATG`
E122A Forward	`CACGGTACTCTATATGATTTGGCATTTTTGGAAAATGACCAAGGT`
E122A Reverse	`ACCTTGGTCATTTTCCAAAAATGCCAAATCATATAGAGTACCGTG`

^a The mutated codon is indicated in bold letters.