FIGURE 6.
Effect of SkzL on pyro-EPR-pNA hydrolysis by Pm. Titrations of the fractional change in initial velocity (Δv/vo) of 10 nm Pm as a function of total wtSkzL concentration ([SkzL]o) in the absence (●) and presence (○) of 10 mm 6-AHA, and as a function of total SkzLΔK415 concentration ([SkzLΔK415]o) in the absence (▴) and presence (Δ) of 10 mm 6-AHA. Solid lines represent the least squares fits of the data by Equation 1 (▴) or Equation 2 (●), with the parameters given under “Results.” The dashed line represents zero. Titrations were performed and analyzed as described under “Experimental Procedures.”