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. 2010 Apr 30;285(27):21153–21164. doi: 10.1074/jbc.M110.107730

FIGURE 7.

FIGURE 7.

Effect of SkzL on Pg activation by uPA. Assays were performed in buffer containing 100 mm chloride (A) or 0.45 mm chloride (B). Initial rates of Pm generation ((nm Pm/s)/(nm uPA)) for activation of 100 nm [Lys]Pg by 0.11 nm uPA in the absence (●) and presence (○) of 10 mm 6-AHA as a function of total SkzL concentration ([SkzL]o) are shown. Initial rates of Pm generation ((nm Pm/s)/(nm uPA)) for activation of 100 nm [Glu]Pg by 0.32 nm uPA in the absence (▴) and presence (Δ) of 10 mm 6-AHA as a function of total SkzL concentration ([SkzL]o) are shown. Solid lines represent the least squares fits of the data by the quadratic binding equation with the parameters listed in Table 4. Experiments were performed and the data analyzed as described under “Experimental Procedures.”