FIGURE 8.

Effect of SkzL on Pg activation by sctPA. Initial rates of Pm generation ((nm Pm/s)/(nm sctPA)) for activation of 100 nm [Lys]Pg by 3 nm sctPA in the absence (●) and presence (○) of 10 mm 6-AHA as a function of total SkzL concentration ([SkzL]_o) are shown. Initial rates of Pm generation ((nm Pm/s)/(nm sctPA)) for activation of 100 nm [Glu]Pg by 3 nm sctPA in the absence (▴) and presence (Δ) of 10 mm 6-AHA as a function of total SkzL concentration ([SkzL]_o) are shown. Solid lines represent the least squares fits by the quadratic binding equation with parameters given under “Results”, and the dashed line represents zero. Experiments were performed and data analyzed as described under “Experimental Procedures.”