FIGURE 9.
Effect of SkzL on uPA- and sctPA-mediated plasma clot lysis. Clot lysis is represented by the time to half-lysis (time to half-lysis) (●) as a function of total SkzL concentration ([SkzL]o) for reactions containing uPA (A) or sctPA (B). Following dilution of all proteins in 50 mm HEPES, 125 mm NaCl, pH 7.4, SkzL was preincubated with 10 mm CaCl2, 7 nm uPA, or 1.5 nm sctPA for 10 min at 37 °C prior to addition of 6 nm thrombin and 160 μl of plasma (200-μl assay volume). Clot lysis was measured by turbidity as described under “Experimental Procedures.” Analysis of clot lysis by uPA and sctPA gave apparent KD values of 1.9 ± 0.9 and 2.5 ± 2.5 μm, respectively.