TABLE 4.
Parameters for the effect of SkzL on Pg activation by uPA
Dissociation constants (KD) for the SkzL-dependent change in the rate of Pm generation for activation of 100 nm [Glu]Pg or [Lys]Pg by 0.11 nm uPA. Experiments were performed in the presence of low and high total chloride ion concentration ([chloride ion]o). Experimental error in the parameters represents ± 2 S.D. Experiments were performed and the results analyzed as described under “Experimental Procedures.”
| Substrate | [chloride ion]o | KD |
|---|---|---|
| mm | nm | |
| [Lys]Pg | 100 | 300 ± 120 |
| [Lys]Pg | 0.45 | 340 ± 150 |
| [Glu]Pg | 100 | 13,000 ± 12,000 |
| [Glu]Pg | 0.45 | 1900 ± 900 |