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Purification of Aro from S22
All kinetics were performed using DCPIP as electron acceptor.
| Purification step | Total proteins | Total activity | Specific activity | Purification | Yield |
|---|---|---|---|---|---|
| mg | μm DCPIP/min | μmol/min/mg | -fold | % | |
| Cell extract | 266.6 | 10.5 | 0.0392 | 1 | 100 |
| DEAE-Sephacel | 11.9 | 9.6 | 0.802 | 20.5 | 91 |
| MonoQ | 1.368 | 5.7 | 4.189 | 106.9 | 55 |
| Gel filtration | 0.684 | 3.9 | 5.7 | 145.4 | 38 |
