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. 2010 Apr 29;285(27):20507–20513. doi: 10.1074/jbc.M109.090027

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Analysis of complementation by Cp-GPI C541S of endogenous ceruloplasmin silencing in C6 cells. Rat glioma C6 cells were silenced for Cp-GPI. After 24 h, they were transfected with Fpn-GFP and human Cp-GPI C541S. Yeast ATPase Ccc2p ability to deliver copper to the mutant Cp was assessed by further co-transfection with recombinant P. pastoris Ccc2p-FLAG construct. Cells were analyzed after 24 h by epifluorescence (Fpn-GFP) or immunofluorescence (hCp). DIC, differential interference contrast.