TABLE 1.

Enzymatic and functional activity of human Cp expressed in P. pastoris

Ceruloplasmin	Oxidase activitya	Specific activity	Functional complementationb
Wild type	0.078	100	+++
R701W	0.053	68	−D
K183W	0.071	91	−
K340W	0.075	96	−
K543W	0.077	99	−
R883W	0.052	67	−
C699S	0.062	79	−D
C618S	0.034	44	−D

^a ΔA₅₄₀/h/μg Cp at 37 °C pH 6 (blank, sample boiled for 5 min).

^b Functional complementation in C6 cells silenced for endogenous Cp-GPI and co-transfected with Fpn-GFP and the corresponding Cp mutant. D, dominant over wild type Cp.