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. 2010 Mar 17;285(27):21103–21113. doi: 10.1074/jbc.M109.080671

FIGURE 4.

FIGURE 4.

Post-translational processing of TNFα. Peritoneal macrophages were isolated from asm+/+ and asm−/− mice (A–C) or from asm+/+ mice (D and E) and treated with LPS (10 ng/ml) for the indicated times. Ammonium chloride (10 mm) was added to the culture medium 1 h after stimulation with LPS as indicated. TNFα levels were determined in cell extracts (A and D) or medium (B, C, and E) by Western blotting. Cyclophilin A (Cycloph. A) levels were used to control for uniform loading. The data shown in A and B were compiled from three different experiments, each covering specific time intervals of stimulation with LPS and each repeated at least twice using different macrophage preparation from two to three mice in each genotype. For quantification purposes, the data were normalized for the respective values in asm+/+ macrophages and are means ± S.D. ***, p < 0.001; *, p < 0.05.