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. 2010 Mar 17;285(27):21103–21113. doi: 10.1074/jbc.M109.080671

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Effect of inhibition of endocytosis on sTNFα secretion in asm^+/+ and asm^−/− macrophages. Macrophages from asm^+/+ and asm^−/− mice were treated for 4 h with LPS (10 ng/ml) in the presence and absence of Dynasore (80 μm), chlorpromazine (2.5 μg/ml), or monodansylcadaverine (MDC) (50 μm) added 1 h after LPS stimulation. sTNFα in the medium was measured by ELISA. Statistical significance of the main effect (***, p < 0.001; **, p < 0.01) and interaction effect (###, p < 0.001) was determined by two-way (for Dynasore treatment) and one-way (for chlorpromazine and monodansylcadaverine treatments) ANOVA.