FIGURE 2.

Analysis of gp120 glycans by mobility shift after endoglycosidase treatment. gp120 expressed in 293T, Jurkat, Dakiki, RD, HepG2, and CHO cell lines were treated with PNGase F, Endo H, or neuraminidase or remained untreated after which the preparations were separated by SDS-PAGE under reducing conditions, blotted on polyvinylidene difluoride membrane, and developed with anti-V5-tag antibody (A), lectins G. nivalis, GNL (high-mannose glycan-specific) (B), A. aurantia, AAL (fucose-specific) (C), or P. vulgaris, PHA-L (specific for complex glycans with ≥3 antennas) (D). Representative data from at least two different experiments are shown.