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. 2010 May 3;285(27):20860–20869. doi: 10.1074/jbc.M109.085472

TABLE 1.

Monosaccharide composition of gp120 produced by different cell lines determined by gas-liquid chromatography

Data are expressed as average from two experiments relative to standard sugars and normalized to internal standard, based on the area under the peak in the chromatograms.

Sugar 293T Jurkat RD HT1080 HepG2 CHO
Fucose 0.21 0.17 0.08 0.20 0.33 0.17
Mannose 3.21 2.19 1.39 2.77 2.16 2.32
Galactose 0.78 0.58 0.42 1.09 1.02 0.63
Glucose 0.19 0.07 0.13 0.07 0.05 0.10
Internal standard 1.00 1.00 1.00 1.00 1.00 1.00
GlcNAc 2.08 1.37 0.59 1.70 1.74 1.27
GalNAc 0.12 NDa ND 0.21 0.13 0.08
Sialic acid 0.59 0.38 0.31 0.63 0.89 0.66

a ND, not detected.