TABLE 2.

Summary of MALDI-TOF and LTQ-FT ICR mass spectrometric analyses of N-glycans of gp120 expressed by different cell lines

Number added to N-glycan corea			293T		Jurkat		RD		HepG2		CHO
			MALDI	FT-ICR	MALDI	FT-ICR	MALDI	FT-ICR	MALDI	FT-ICR	MALDIb	FT-ICR
Hex	GlcNAc	Fuc	Errorc	Error	Error	Error	Error	Error	Error	Error	Error	Error
High-mannose glycans
2	0	0	0.675	0.004	0.498	−0.007	0.582	0.008	0.690	−0.002	0.089	0.002
3	0	0	0.750	−0.005	0.540	−0.006	0.660	−0.003	0.773	−0.001	0.109	0.000
4	0	0	0.634	0.000	0.569d	−0.003	0.628	0.003	0.761	0.001	0.121	0.003
5	0	0	0.787	0.004	0.601	−0.004	0.685	0.003	0.817	0.000	0.128	−0.001
6	0	0	0.840d	0.002	0.649	−0.003	0.742	−0.001	0.880	0.003	0.137d	0.000
Complex/hybrid glycans
1	1	0							0.958	0.000	0.101	0.001
1	1	1	0.751				0.657		0.771		0.110	0.000
2	1	0	0.752				0.657		1.043		0.106
2	1	1	0.802	0.002	0.616	0.001	0.737		0.833
3	1	0	0.798	−0.002
3	1	1	0.838	−0.005	0.780	0.000
0	2	1	0.749				0.667
1	2	0	0.727				0.674		0.818		0.107
1	2	1	0.807	0.001	0.687		0.698	0.000	0.844	0.005	0.126	−0.002
2	2	0	0.821			−0.001	0.703	−0.002	0.839	0.003	0.124	−0.002
2	2	1	0.876	−0.005	0.694	0.001	0.739d	0.003	0.895	−0.004	0.138	−0.002
2	2	2							0.930d	−0.003
3	2	0	0.883	−0.002			0.752	0.008	0.920	0.000	0.115
3	2	1	0.912								0.139	−0.006
4	2	0		0.009
0	3	1		−0.001							0.093
1	3	1	0.867d	−0.006			0.768	0.003		−0.002	0.134
2	3	0	0.870				0.735			0.005
2	3	1	0.919	−0.001	0.716		0.768	0.009	0.948	−0.004
2	3	2								−0.001
3	3	0	0.895	−0.008		0.000	0.792		0.942	−0.005	0.127	−0.002
3	3	1	0.974	−0.009		−0.006	0.873	0.005	1.000d	−0.006	0.142	−0.004
3	3	2							1.099	−0.006
0	4	1					0.861	0.006
1	4	1	0.954	−0.009
2	4	1	0.968	−0.005	0.785	−0.001	0.918			−0.009	0.102
3	4	0	1.042
3	4	1	1.011		0.775	−0.001			1.093	0.001
4	4	0	0.931	−0.006					0.992	0.010
4	4	1	1.111		0.801	−0.002	0.968		1.095	−0.003	0.141
4	4	2							1.144
3	5	1	1.112								0.268
4	5	1	1.133

^a N-glycan core is (GlcNAc)₂(Man)₃.

^b Analyzed by MALDI-TOF/TOF.

^c For each glycan detected, error in daltons is shown. The empty lanes means that the glycan was not detected in the particular gp120 preparation.

^d Denotes glycans with tandem mass spectra shown in supplemental Fig. S2. Glycans from each gp120 sample were analyzed twice. Relative abundance data from FT-ICR MS spectra are shown in supplemental Table S1.