FIGURE 3.
Cell adhesion to PIIBNP is integrin αv-dependent. A, αv integrin siRNA-treated and control siRNA-treated hCh-1 cells were lysed, and mRNA level was detected by a semiquantitative PCR method as described under “Experimental Procedures.” B, siRNA-treated and control siRNA-treated hCh-1 cell membrane proteins were solubilized in cell lysis buffer containing Triton X-100. Cell lysate was resolved by SDS-PAGE and integrin αv expression was analyzed by Western blotting. C, siRNA-treated (white bar) and control siRNA-treated (black bar) hCh-1 cell adhesion to GST, PIIBNP, and mutant PIIBNP were assayed as described above. The value of absorbance at 595 nm represents the number of cells bound to the proteins. Absorbance values for siRNA-treated (white) and control hCh-1 cells (black) are represented as mean ± S.D. (*, p < 0.001 compared with control siRNA-treated cells; n = 8).