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. 2010 Apr 20;285(27):20625–20633. doi: 10.1074/jbc.M109.049700

FIGURE 4.

FIGURE 4.

Tandem affinity purification of GABAB receptors from transgenic mouse brains. A, left panel, immunoblot of NTAP-GABAB1a purification steps stained with an anti-HA antibody; post STV, extract after incubation with the streptavidin (STV) matrix; STV eluate, protein eluted with biotin from the STV matrix. A, right panel, immunoblot of GABAB1a/b-CTAP purification steps stained with an anti-GABAB1 pan antibody (AB1531). post IgG, extract after incubation with the IgG matrix; pre TEV, IgG-bound protein prior to TEV protease cleavage; post TEV, eluate of the IgG matrix after incubation with TEV protease. Anti-GABAB1 AB1531 strongly reacted with protein A revealing primarily the TAP-tagged variants (compare with Fig. 2B). B, immunoblots of input and final TAP fractions prepared from the transgenic lines (tg) and wild-type (wt) control animals. B2, GABAB2; PSD-95, postsynaptic density protein 95.