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. 2010 Apr 28;285(27):20691–20703. doi: 10.1074/jbc.M109.086470

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FIGURE 4. — Chemical rescue of the activity of SSO1353 mutants. (A) Dependence of activity of D462G mutant on different concentrations of sodium azide. (B) TLC analysis of the reaction mixtures of SSO1353 wild type and mutants in the absence (lanes 1–6) and in the presence of 0.1 m sodium azide (lanes 8–13). Standard assays were performed overnight at 65 °C on 40 mm 2NP-Glc by using 11 μg of enzyme. Lane 1, blank with no enzyme; lane 2, wild type; lane 3, E335G mutant; lane 4, D462G; lane 5, D458G; lane 6, D406G; lane 7, standards (2NP-Glc and Glc); lane 8, blank with no enzyme; lane 9, wild type; lane 10, E335G; lane 11, D462G; lane 12, D458G; lane 13, D406G; lane 14, β-glucosyl-azide standard.