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Multi-alignment of SSO1353 with glucosylceramidases. Invariant residues are indicated with “*”; increased level of conservation is indicated with “:” and “.” The residues corresponding to the nucleophile Glu-335 and acid/base Asp-462 of SSO1353 are boxed. Pan is XP_001167952.1 from Pan troglodytes; Homo is NP_065995.1 from Homo sapiens; Ciona is XP_002127036.1 from Ciona intestinalis; Sulfolobus is SSO1353 from S. solfataricus P2.
