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The effect of divalent metal ions (100 μm) on the dissociation of heme from the Hx-heme complex (4.3 μm) in ammonium bicarbonate buffer (20 mm, pH 7.4 (adjusted with dilute HCl), 25 °C). (□) no additions, (♦) MnCl2, (▾) CoCl2, (▴)NiCl2, (●) CuCl2, or (■) ZnSO4.
