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The effect of anions on dissociation of heme from the Hx-heme complex (4.1 μm) in ammonium salt buffers (pH 7.9 ± 0.1, 15 °C). (□) ammonium bicarbonate buffer (20 mm), (◇) ammonium bicarbonate (10 mm) plus ammonium chloride (10 mm); (▿), ammonium phosphate (10 mm NH4H2PO4, pH adjusted with ammonium hydroxide); (○), ammonium chloride (20 mm, pH adjusted with ammonium hydroxide); and (◁) tetramethylammonium chloride (20 mm (CH3)4N(OH), pH adjusted with HCl).
