FIGURE 2.

Effect of XOMA 052 on IL-1β binding to receptors. XOMA 052 reduces the affinity of IL-1β binding to sRI measured by KinExA from 2 to 10 nm (n = 5) (a) but has no effect on the affinity of IL-1β binding to sRII of 2 nm (n = 2) (b). XOMA 052 reduces the affinity of sRAcP binding to sRI·IL-1β complex from 25 (c) to 69 nm (d), as measured by SPR. XOMA 052 shifts the apparent affinity of Alexa Fluor 647-labeled IL-1β binding to HEK293 cells expressing IL-1RI and IL-1RAcP from 2 to 9 nm (n = 2) (e).