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. 2010 Apr 7;285(27):21092–21102. doi: 10.1074/jbc.M110.104836

FIGURE 5.

FIGURE 5.

Effect of mutations in CLDN1 and CLDN7 EC1 on CD81 and Occludin association. 293T cells were transfected to express AcGFP (g) and DsRED (r) fluorescence-tagged wild-type and mutant forms of g.CLDN and r.CD81 (A) or r.Occludin (B), and the degree of association between fluorophore-tagged proteins was assessed by FIR and FRET analysis. C, 293T cells were transfected with AcGFP- and DsRED-tagged versions of wild-type and mutant CLDN constructs to assess the effect of EC1 mutations on CLDN-CLDN cis-interactions. Median FIR and FRET values from ten individual cells are presented (*, p < 0.05; **, p < 0.01).