FIGURE 3.

Expression of the tan gene in wild type C-S, tan¹, and tan⁵ heads. A–C, immunolabeling of Tan in wild type (A), tan¹ (B), and tan⁵ (C) 10 μm Drosophila head freeze sections with anti-Tan antiserum ap61/ap63 (5). The Tan label is in blue. Ebony labeled in green is used as control. Re, La, and Me indicate retina, lamina, and medulla, respectively. D, Western blot analysis of wild type Canton-S (C-S) and mutants tan¹ (t¹) and tan⁵ (t⁵) head extracts with anti-Tan antiserum. E, RT-PCR amplification of a 456-nucleotide tan-RNA fragment (strong lower band) from head total RNA preparations of wild type Canton-S (C-S) and mutants tan¹ (t¹) and tan⁵ (t⁵). RT-PCR amplification of a 581-nucleotide ebony-RNA fragment (weak upper band) served as control.