TABLE 1.

Pseudomonas putida and Escherichia coli strains and plasmids used in this study

Strain or plasmid	Characteristicsa	Source/Ref.
Strains
P. putida DOT-T1E	TolR, RifR	12
P. putida DOT-T1E13	RifR, KmR, ttgR::&OHgr;Km	15
E. coli (BL21 DE3)		Novagen
Plasmids
pMP220	TcR, lacZ promoter fusion vector	18
pET29▵+)	KmR, T7 protein expression vector	Novagen
pWilR2	KmR, pET29▵+) with wild-type ttgR	10
pETttgRL66A	KmR, pET29▵+) with L66A mutant ttgR	This study
pETttgRH67A	KmR, pET29▵+) with H67A mutant ttgR	This study
pETttgRH70A	KmR, pET29▵+) with H70A mutant ttgR	This study
pETttgRH72A	KmR, pET29▵+) with H72A mutant ttgR	This study
pETttgRR75A	KmR, pET29▵+) with R75A mutant ttgR	This study
pETttgRV96A	KmR, pET29▵+) with V96A mutant ttgR	This study
pETttgRR130A	KmR, pET29▵+) with R130A mutant ttgR	This study
pETttgRF168A	KmR, pET29▵+) with F168A mutant ttgR	This study
pETttgRR176G	KmR, pET29▵+) with R176G mutant ttgR	16
pETttgRL66AV96A	KmR, pET29▵+) with L66AV96A mutant ttgR	This study
pWtttgR-A::lacZ	TcR, wild-type ttgR-ttgAp::lacZ promoter fusion	This study
pL66AttgR-A::lacZ	TcR, L66A ttgR-ttgAp::lacZ promoter fusion	This study
pL66AV96AttgR-A::lacZ	TcR, L66A ttgR-ttgAp::lacZ promoter fusion	This study
pH67AttgR-A::lacZ	TcR, H67A ttgR-ttgAp::lacZ promoter fusion	This study

^a Tol^R, Rif^R, Km^R, and Tc^R stand for resistance to toluene, rifampicin, kanamycin, and tetracycline, respectively. ttgA^P refers to the promoter in front of the ttgA that is fused to the promoterless lacZ gene.