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. 2010 May 7;285(28):21411–21415. doi: 10.1074/jbc.M110.103564

TABLE 1.

A. thaliana CAT2 mutant alleles

Accession number: AtCAT2: AT4G35090.1.

Allele name Amino acid position Amino acid Mutant amino acid HU survivala Catalase activityb S.E.
% of wt
cat2-1c 4th exond 76 19.3 2.4
cat2-2e 3rd exond 79 14.1 4.4
cat2-3 61 E K 81 29.9 3.9
cat2-4 73 G D NDf ND ND
cat2-5 104 S F 57 29.6 2.1
cat2-6 111 G E 37 29.4 4.0
cat2-7 114 E K 82 59.0 7.1
cat2-8 137 G R 78 40.6 5.2
cat2-9 182 S N 69 19.0 4.2
cat2-10 185 M I 28 38.8 2.6
cat2-11 251 A T 73 15.7 0.7
cat2-12 265 P L 80 21.8 2.4
cat2-13 267 W Stop 70 49.4 7.1
cat2-14 293 W Stop 72 38.3 5.4
cat2-15 332 G R 99 18.6 3.3
cat2-16 344 R Q 97 30.2 5.2
cat2-17 450 W Stop 94 23.6 1.8
cat2-18 473 No splice 93 31.3 3.4
cat2-19 480 Q Stop 80 21.2 3.7

a Average germination rate for plants grown on 5, 10, and 25 mm HU. For wild-type plants, this value is zero.

b All measured mutant catalase activities were significantly lower than that of the wild type (95% CL, 1-way analysis of variance, Tukey post-test, GraphPad Prism 5.0).

c SALK_076998 T-DNA line.

d As described (20).

e SALK_057998 T-DNA line.

f ND, not determined.