TABLE 1.
Allele name | Amino acid position | Amino acid | Mutant amino acid | HU survivala | Catalase activityb | S.E. |
---|---|---|---|---|---|---|
% of wt | ||||||
cat2-1c | 4th exond | 76 | 19.3 | 2.4 | ||
cat2-2e | 3rd exond | 79 | 14.1 | 4.4 | ||
cat2-3 | 61 | E | K | 81 | 29.9 | 3.9 |
cat2-4 | 73 | G | D | NDf | ND | ND |
cat2-5 | 104 | S | F | 57 | 29.6 | 2.1 |
cat2-6 | 111 | G | E | 37 | 29.4 | 4.0 |
cat2-7 | 114 | E | K | 82 | 59.0 | 7.1 |
cat2-8 | 137 | G | R | 78 | 40.6 | 5.2 |
cat2-9 | 182 | S | N | 69 | 19.0 | 4.2 |
cat2-10 | 185 | M | I | 28 | 38.8 | 2.6 |
cat2-11 | 251 | A | T | 73 | 15.7 | 0.7 |
cat2-12 | 265 | P | L | 80 | 21.8 | 2.4 |
cat2-13 | 267 | W | Stop | 70 | 49.4 | 7.1 |
cat2-14 | 293 | W | Stop | 72 | 38.3 | 5.4 |
cat2-15 | 332 | G | R | 99 | 18.6 | 3.3 |
cat2-16 | 344 | R | Q | 97 | 30.2 | 5.2 |
cat2-17 | 450 | W | Stop | 94 | 23.6 | 1.8 |
cat2-18 | 473 | No splice | 93 | 31.3 | 3.4 | |
cat2-19 | 480 | Q | Stop | 80 | 21.2 | 3.7 |
a Average germination rate for plants grown on 5, 10, and 25 mm HU. For wild-type plants, this value is zero.
b All measured mutant catalase activities were significantly lower than that of the wild type (95% CL, 1-way analysis of variance, Tukey post-test, GraphPad Prism 5.0).
c SALK_076998 T-DNA line.
d As described (20).
e SALK_057998 T-DNA line.
f ND, not determined.