TABLE 1.

RT-PCR primers

Primers and conditions used to detect multiple cWnt signaling molecules as depicted in Fig. 1A. The RT-PCR primers were designed to recognize mouse sequences.

Target	Primer sequence		Size	Annealing temperature	Cycle #	Ref.
	Sense	Antisense
Wnt1	AAATCGCCCAACTTCTGCA	AATACCCAAAGAGGTCACAGC	599	53.2	40	16
Wnt2	CGGCCTTTGTTTACGCCATC	TGAATACAGTAGTCTGGAGAA	513	55.3	40	16
Wnt2b	TGTACTCTGCGCACCTGCT	TGCACTCACACTGGGTGAC	318	60.0	40	16
Wnt3a	ATTGAATTTGGAGGAATGGT	CTTGAAGTACGTGTAACGTG	317	57.5	40	16
Wnt4	TGTACCTGGCCAAGCTGTCAT	TCCGGTCACAGCCACACTT	344	60.0	40	16
Wnt5a	TCCTATGAGAGCGCACGCAT	CAGCTTGCCCCGGCTGTTGA	224	60.0	40	16
Wnt5b	TCGGAGGAGCAGGGCCGAGC	CAGCTTGCCCTGGCGGGTGA	223	65.0	40	16
Wnt6	GCACCGAGTGTAAGTGCCAT	GAAGCGGCACAGACAGTTCT	377	56.2	40	16
Wnt7a	CAAGGCCAGTACCACTGGGA	GGCTCCACGTGGACGGCCTC	307	56.5	40	16
Wnt7b	ACCAAAACTTGCTGGACCAC	ACGTGTTGCACTTGACGAAG	385	56.2	40	16
Wnt8b	AACGTGGGCTTCGGAGAGGC	GCCCGCGCCTTGCAGCAGGT	272	62.9	40	16
Wnt9b	GTAAGTGCCATGGTGTGTC	GTGTCATAGCGTAGCTTCAG	160	55	28	17
Wnt10a	AAAGTCCCCTACGAGAGCCC	CAGCTTCCGACGGAAAGCTT	179	52.3	40	16
Wnt10b	CGGCTGCCGCACCACAGCGC	CAGCTTGGCTCTAAGCCGGT	179	59.3	40	16
Wnt11	GTGGCTGCTGACCTCAAGACC	TTCTTCATGCAGAAGTCAGGAG	103	55	28	17
Wnt16	ACAGCATCCAGATCTCAGAC	ACTACATGGGTGTTGTAGCC	238	55	28	17
Frz2	CTAGCGCCGCTCTTCGTGTACCTG	CAGCGTCTTGCCCGACCAGATCCA	387	60	25	18
Frz3	TAGCAATGGAGCCCTTCCACC	CTCCATATCTTCAGGCCACGG	200	55	28	17
Frz4	GCTTGTGCTATGTTGGGAACCCAC	ACAGGTTGCAGGAACCGT	230	55	28	17
Frz5	GTCTGTGCTGTGCTTCATC	AGTGACACACACAGGTAGCA	123	55	28	17
Frz6	TGAAGGAGAGAAGCAATGGATC	TGAACAGGCAGAGATGTGGAG	160	55	28	17
Frz7	GCCAGACCCACCTTTCACT	CGAACCGTCTCTCCTCTTCTT	198	58	35	19
Frz8	TTTGTGCTGGCGCCACTGGTT	TAGAGCACGGTGAAGAGGCCC	170	55	28	17
Frz9	CGGAGTCTTTTCCATCCTTTACAC	GCCATTTTTCGGTAGCACAGG	250	58	35	20
Frz10	TGTCCGGTTGCTACACCATGGGCT	GCCAGGAACCAGGTGAGG	220	55	28	17
LRP5	GATGTGCGGCTAGTGGATG	GCCCGAGATGACAATGTTCT	195	58	35	21
LRP6	GGGCCGATGCAAAACTTAAT	CCTCTGTTGGCTGAAAGCAT	242	58	35	21
Dsh1	GGGGGTAGTGGCAGTGAA	ACCTGTAAGTTCTGGAGGGACA	237	58	35	21
Dsh2	GCAGTGGCAGTGAGTCAGAA	TCATGGGGTTATAGGGGAGAG	192	58	35	21
Dsh3	CAAGGAGAAGGACCCAAAAG	ATCGGGGGACCATAGAGAG	240	58	35	21
APC	TCACCTAGCGGGACTGTTG	CTTCTTCGTGTTGGTGCTCA	132	55	40	Roel Nusse, The Wnt Homepage (Stanford, Palo Alto, CA)
Axin1	CAGGGTTTCCCCTTGGACC	GGTCAAACATGGCAGGATC	535	58	30	22
Axin2	CTCCTTGGAGGCAAGAGC	GGCCACGCAGCACCGCTG	657	58	40	23
GSK3β	CAGCAGCCTTCAGCTTTTGG	CCGGAACATAGTCCAGCACCAG	358	60	35	24
Rspo1	TGTGAAATGAGCGAGTGGTCC	TCTCCCAGATGCTCCAGTTCT	416	58	32	25
Rspo2	TTGCATAGAGGCCGCTGCTTT	CTGGTCAGAGGATCAGGAATG	501	58	32	25
Rspo3	GTACACTGTGAGGCCAGTGAA	ATGGCTAGAACACCTGTCCTG	474	58	32	25
Rspo4	CTGGAGTCCCTGCATACACAA	CACGGGGAGAAGGAAAGTTTC	439	58	32	25