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. 2010 May 7;285(28):21446–21457. doi: 10.1074/jbc.M109.090043

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Inhibition of Hck-T338A kinase activity with NaPP1 selectively restores the apoptotic response to imatinib in K562-Hck-T338A cells. K562-Neo, K562-Hck, and K562-Hck-T338A cell populations were incubated with the indicated combinations of imatinib and NaPP1 for 72 h. Apoptotic cells were stained with an anti-phosphatidylserine antibody conjugated to Alexa Fluor 488, and the percentage of apoptotic cells was determined by flow cytometry. The apoptotic response to imatinib was plotted for each individual NaPP1 concentration. Each point represents the average percentage of apoptotic cells generated from three independent experiments ±S.D. Two-way ANOVA was performed to determine statistical significance of the effect of imatinib and NaPP1 on each individual cell line. For K562-Neo and K562-Hck cells, the apoptotic effect of imatinib was statistically significant with p < 0.0001, whereas NaPP1 had no significant effect (p > 0.05). For K562-Hck-T338A cells, both imatinib and NaPP1 had a significant effect (p < 0.0001).