FIGURE 7.

cPLA₂ activation in Chlamydia-infected MEFs is mediated by the MAPK p38. A, MEFs were infected with C. trachomatis and treated with inhibitors of the ERK (U0126) or p38 (SB203580) MAPK pathways and the levels of activated signaling and IRG proteins were determined by immunoblots. Note that inhibition of p38, but not ERK, completely abolished cPLA₂ phosphorylation at Ser-505 and expression of the IRG protein Irgb6. B, MEFs transduced with an IFN-inducible luciferase reporter were treated with ERK or p38 inhibitors and the levels of luciferase expression in response to C. trachomatis infection was determined.