FIGURE 2.

Reconstitution of IRF-8 in IRF-8-deficient BMDMs. 1 × 10⁶ bone marrow cells isolated from femurs of IRF-8^−/− mice were inoculated into each well of 24-well plates with 1 ml of RPMI 1640 complete medium with 10 ng/ml M-CSF and 10% fetal bovine serum. At day 2 and day 4, the cells were infected with retroviruses encoding IRF-8 or green fluorescent protein for two rounds. The transduced BMDMs and BMDMs differentiated from WT mice were treated with LPS or IFN-γ plus LPS for 4 h. Then total RNA was extracted from the activated BMDMs, and 1 μg of RNA was used for reverse transcription, followed by measurement of mouse IL-27 p28 (A) and IRF-8 (B) mRNA expression by qRT-PCR. Data are representative of two experiments with similar results. UD, undetectable. Error bars, S.D.