FIGURE 6.

CaMKIIδ mediates low concentration thrombin-induced RhoA activation. A, RhoA activation (G-LISA RhoA activity, Cytoskeleton Inc., Denver, CO) in HUVEC monolayers in response to 2.5 nm and 50 nm addition of thrombin for the indicated times. RhoA activity is expressed as fold changes relative to untreated controls. B, effect of siRNAs targeting CaMKIIδ (siCaMKIIδ) on thrombin stimulated RhoA activation. Control cells without siCaMKIIδ were treated with scrambled control siRNA (siCon). Data values are means ± S.E. from five independent experiments. *, p ≤ 0.05 effects of thrombin on RhoA activity; #, p ≤ 0.05 compared between siRNA groups; n.s., no significant differences.