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. 2010 May 4;285(28):21607–21614. doi: 10.1074/jbc.M110.122788

TABLE 1.

Dissociation constants (Kd) and association and dissociation rate constants (kassoc and kdiss) for the interaction of horse, snake, and yeast Cyt c, and G. brevicaudus basic PLA2 with immobilized human LRG or G. brevicaudus PLIβ

Ligand Analyte Kd kassoc kdiss
m m1s1 s1
Human LRG Horse Cyt c 1.58 ± 9.92 × 10−13 2.00 ± 0.02 × 106 3.15 ± 19.8 × 10−7
Snake PLIβ Horse Cyt c 1.05 ± 0.19 × 10−10 2.53 ± 0.05 × 107 4.18 ± 0.08 × 10−3
Human LRG Snake Cyt c 1.65 ± 0.06 × 10−10 3.90 ± 0.36 × 107 4.08 ± 0.35 × 10−3
Snake PLIβ Snake Cyt c 2.37 ± 0.48 × 10−12 7.86 ± 0.12 × 106 1.86 ± 0.35 × 10−5
Human LRG Yeast Cyt c NBa NB NB
Snake PLIβ Yeast Cyt c 1.67 ± 0.05 × 10−6 1.26 ± 0.04 × 103 2.10 ± 0.01 × 10−3
Human LRG Basic PLA2 NB NB NB
Snake PLIβ Basic PLA2 1.21 ± 0.03 × 10−9 8.69 ± 0.13 × 105 1.05 ± 0.01 × 10−3

a NB, no binding.