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Temperature and pH dependence of SGP activity. Effect of temperature on the SGP pH profile of hydrolysis of the substrate MCA-KLFSSKQ-EDDnp (A). The kcat/Km values were determined at pseudo first-order reactions measured in 100 mm citric acid-phosphate buffer at 10 (○), 20 (▴), 30 (□), and 40 °C (●). B, the van't Hoff plot for SGP pKe1 (○) and pKe2 (●) temperature dependence.
