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Proton inventory of the specificity constant kcat/Km (●) and kcat (○) for SGP hydrolysis of MCA-KLFSSKQ-EDDnp. Ratios of kcat/Km and kcat measured in mixed isotopic solvents over the kcat/Km and kcat measured in water were plotted against the atom fraction of deuterium, n. Assay conditions are described under “Experimental Procedures.” Bars are S.D. from triplicate experiments.
