FIGURE 1.

Detection of mitochondrial SIRT1 and PGC-1α by immunofluorescence microscopy in SH-SY5Y cells. A, mitochondrial network and nuclei were evidenced by incubation with mouse anti-cytochrome c and Hoechst 33342, respectively. PGC-1α or SIRT1 was stained by using rabbit antibodies (Santa Cruz Biotechnology). B, PGC-1α and SIRT1 were stained by using goat anti-PGC-1α (Santa Cruz Biotechnology) and rabbit anti-SIRT1. Cells were analyzed by an Olympus Delta vision deconvolution fluorescent microscope. The yellow-orange color obtained by image merge indicates the regions where the green and red signals superimpose. White arrows indicate some regions of co-localization signals. Scale bar, 10 μm.