FIGURE 2.

CRIF1 interacts with both N- and C-terminal sequences of NRF2. A, IVT CRIF1 and FLAG-NRF2 proteins interact. ³⁵S-Labeled proteins (see “Experimental Procedures”) were mixed, immunoprecipitated with an anti-FLAG antibody, subjected to SDS-PAGE, and exposed to x-ray film. These experiments were repeated three times and yielded similar results; a representative result is shown. ³⁵S-Labeled IVT luciferase was used as the negative control. B, schematic of the NRF2 deletion mutants used for the experiments in C. C, CRIF1 interacts with both N- and C-terminal regions of NRF2. Mixtures of unlabeled IVT full-length FLAG-CRIF1 and three ³⁵S-labeled NRF2 deletion mutants were immunoprecipitated with the anti-FLAG antibody, subjected to SDS-PAGE, and exposed to x-ray film.