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Artificial Muc21/epiglycanin cDNA having 84 TR was constructed and expressed in 293T cells. A, methods of constructing the Muc21 expression vector are summarized. B, cells transiently transfected with the Muc21-IRES2-Venus vector were stained with mAb 1A4-1 and analyzed by flow cytometry using allophycocyanin-conjugated streptavidin as a fluorescence group at an excitation wavelength 638 nm and emission wavelength 660/20 nm.
