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. 2010 Apr 13;285(28):21233–21240. doi: 10.1074/jbc.M109.082875

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Long TR domain seemed to have a major effect on the antiadhesive property of Muc21. A, mutants lacking a cytoplasmic domain were constructed with an N-terminal FLAG tag and transfected into 293T cells. The number of floating cells in FLAG-positive cells was evaluated. B, extracellular domain mutants having various portions of Muc21 were constructed with an N-terminal FLAG tag and C-terminal c-myc tag and transfected into 293T cells. Note that the mutants in B were constructed using a different vector system from the mutants in A.