FIGURE 6.

α-Syn binds to both β-arrestin and RalGDS, and the interaction of β-arrestin and RalGDS is enhanced by α-syn overexpression. A, after transfection of HUVECs with GFP or GFP-α-syn and treatment with 100 nm PMA for 1 h, the cells were lysed and immunoprecipitated with anti-GFP and anti-β-arrestin antibodies. B, anti-GFP and anti-RalGDS antibodies; D, anti-RalGDS and anti-β-arrestin antibodies. con, control. After transfection of HUVECs with GFP, GFP-syn(1–95), or GFP-syn(96–140) and treatment with 100 nm PMA for 1 h, the cells were lysed and immunoprecipitated with anti-GFP (C) or anti-β-arrestin antibodies (E), and the immunoprecipitated proteins were analyzed by Western blot for the indicated antibodies.