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. 2010 May 6;285(28):21416–21425. doi: 10.1074/jbc.M110.103499

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — α-Syn is localized close to WPB in HUVECs. HUVECs were prepared as described under “Experimental Procedures,” and the sections were incubated overnight in the presence (A–C) or absence (D and E) of α-syn antibody at 4 °C and then with 10-nm gold conjugated goat anti-mouse IgG antibody. The sections were observed under an electron microscope. The regions of A and D in the boxes were magnified in the images of B, C and E, F, respectively. Note that α-syn was close to WPB in HUVECs. Arrow indicates the cluster of gold particles. Scale bar indicates 0.6 μm.