FIGURE 6.

Immortalized FAK R454 MEFs exhibit motility and polarity defects. A, wound closure motility of WT and R454 MEFs was calculated by measuring the distance change between 0 and 16 h in time lapse experiments. Values are means ± S.D. from 10 independent analyses. B, chamber motility was measured over 4 h with WT and R454 MEFs with FN and serum. Values are means ± S.D. from three experiments. C, FAK activity is required for Golgi reorientation and cell polarization. 100 cells were analyzed, and values represent percentage of cells ± S.D. D, representative images from Golgi reorientation assay. WT and R454 MEFs were grown to confluence, wounded with a pipette tip, and allowed to migrate in the presence of serum for 4 h. Cells were fixed, imaged in phase, and stained for Golgi (β-Cop, red) and nuclear (Hoechst, blue) markers. Position of the leading lamella (dashed line) is indicated. Bar, 10 μm.