FIGURE 2.

Induction of endozepine production by KCl. Astrocytes in 24-well plates were washed twice with HSCC buffer and incubated in 500 μl of HSCC buffer at 37 °C. Endozepine production was induced by adding 50 mm KCl to the astrocytes (triangles). 2 μm TPCK (open squares) or anti-ACBP antibodies (diamonds) diluted 1/5,000 were added just prior to induction by 50 mm KCl. At the indicated times, 400 μl of buffer was harvested to quantify the amount of endozepines using the Dictyostelium bioassay. Each experiment was repeated at least three times. To standardize the experiments, cells from a well in each series were lysed using Triton X-100 and the amount of soluble protein measured. The bars indicate the reliability range.