Figure 3.

Crystals and a sample diffraction image from PPM cystallized with Mn²⁺. (a) Crystals of PPM grown in the presence of 50 mM MnCl₂. Optimized crystals of PPM that were identifed after supplementing the protein with 50 mM MnCl₂. Crystals grew from 100 mM bis-tris pH 5.5, 50 mM MnCl₂, 13–16% PEG 3350 and 25–100 mM ammonium acetate and appeared in 3–5 d at 291 K. The black bar represents 100 µm. (b) Diffraction from crystals of PPM grown in the presence of 50 mM MnCl₂. Diffraction images collected at APS on beamline ID-21-G using 1° oscillations per frame revealed that these crystals belonged to space group P2₁. A data set consisting of 180 frames and is 98.6% complete to 1.85 Å was collected.