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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: J Invest Dermatol. 2010 Jan 7;130(5):1384–1390. doi: 10.1038/jid.2009.416

Figure 5. Piasy regulates CCL20 through p65/RelA subunit of NF-κB.

Figure 5

a) CCL20 mRNA was measured in 291 keratinocytes transfected with plasmids expressing GFP, p65, Trim32 plus p65, or Piasy plus p65. Data are representative of two independent experiments. Statistical analysis of Trim32 and Piasy effect was conducted relative to p65 alone control (*, P< 0.05). b) Piasy interacts with endogenous p65 in keratinocytes. Flag-tagged Piasy and Flag-tagged Erk (negative control) were transfected into 291 keratinocytes. The bound p65 was immunoprecipitated with an anti-Flag antibody and visualized by immunoblotting with anti-p65 antibody.