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. Author manuscript; available in PMC: 2011 May 25.
Published in final edited form as: Biochemistry. 2010 May 25;49(20):4349–4360. doi: 10.1021/bi100215b

Figure 1.

Figure 1

Expression and purification of GSNL-1 variants. (A) Schematic representation of domain structure of full-length GSNL-1 (top). G domains are designated as G1 to G4. Positions of N- and C-termini of each domain are shown on top. Positions of amino acid sequence for each GSNL-1 fragments are shown. (B) Purified recombinant GSNL-1 variants (0.5 μg each) were subjected to SDS-PAGE and stained by Coomassie Brilliant Blue. Molecular mass markers (M) in kDa are shown on the left of the gel.