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Effects of GSNL-1 variants on critical concentration of actin. Varying concentrations (0.1 – 1.0 μM) of 20 % pyrene-labeled actin were polymerized with or without 50 nM of GSNL-1 variants in the presence of 0.1 mM CaCl2 (A) or 5 mM EGTA (B). After overnight incubation, the pyrene fluorescence (arbitrary units) was measured and plotted as a function of total actin concentration.
